For example, the Spearman’s rank correlation coefficient comparing reference manual to automated analysis for the 14 Basic panel cell populations were all > 0.8. Automated analyses produced results that were highly correlated with those obtained in the reference manual analysis. In addition, we compared the reference manual values with those obtained by 2 additional manual analyzers who followed an identical gating strategy. The performance of automated pipelines was assessed by their ability to match on a per-event basis values obtained by an expert manual analyzer (i.e., the reference manual), currently considered the “gold standard” approach. pre-formatted panel antibody cocktails technology (Beckman Coulter). Data was generated using DuraClone’s dry reagent. We developed an automated analysis workflow based on flowCore and flowDensity. Implementation of Automated Gating Strategies for Quality Control and Analysis of Checkpoint Marker, PD-L1, in Hematologic Malignancies.
This study was presented by: Alberto Hidalgo Robert, Shadi Eshghi, Ryan Brinkman, Sibyl Drissler, Daniel Yokosawa, Cherie Green, W. With computational analysis, each sample took approximately 60 seconds – and that is hands-off computer time. Automated methods decreased hands-on analysis time.It showcased the feasibility of using computational gating and reproducible quality assessment and analysis. This study demonstrated that computational analysis provides a standardized pipeline mirroring manual analysis.The variability between automated and manual analysis is comparable with inter- and intra- operator variability observed on the same dataset.Lower scores did not reflect poor performance of automated methods the manual analysis of these populations was more subjective and thus a challenge to match with data-driven thresholds.Heterogeneous and low frequency populations, such as PD-L1+CD34+ CD45dimBlasts, yielded slightly lower, yet acceptable concordance with manual analysis (0.75 Results showed a high concordance of cell count and percentage (of parent gate) between manual and automated analysis, particularly in high frequency and homogenous populations, such as lymphocytes (0.90
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